Contact an Account ManagerDetermination of hesperidin in orange juice and orange soft drinks by HPLC with an amperometric detector
Summary
Type: HPLC
Application #: 107000
Catalog#: 720013.40 - MN HPLC Column, Nucleosil C8, 100A, 5um, 4x250mm
Detection: electrochemical, glassy carbon electrode, +1.3V
Conditions: eluent: methanol - acetic acid - water (350:25:625, v/v) adjusted to pH 3 with NaOH
flow rate 0.9ml/min
Authors: Sontag G
Source: Z Anal Chem 309 (1981) 109 - 113
Details
Legend: Fig. 3. Chromatogram of an orange juice, 1. hesperidin, 2. vanillic acid methyl
ester (int. std.).